FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372261
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.039.4757548
GTCTCGC8750.039.2083051
GTCTTGC17350.037.0229871
TCGCTCT9000.032.2189454
GATCCGT2051.1401426E-831.8287146
GGGTACG1453.334134E-530.2044931
CTGTCGC13500.028.9994959
CGCCGTT2054.041467E-728.29398145
GTGTAAG9300.027.470931
GTATTAT4800.027.372821
CTCGCTC10100.027.276753
GTATCGG1357.7713956E-427.0348831
CCGTGTT5900.027.0334269
GTATTAA5500.026.543341
TATACTG5200.026.4893655
TTAGTCG1100.00967602726.362625
GTCTTAC6650.026.3437671
GTCTTGT9200.026.1826971
GTATAAT5750.025.389281
GTCCGAA1450.00117929625.170411
Produced by FastQC (version 0.11.5)