FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281365
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCC6850.043.39184145
TCTGTCG8500.040.0896038
TAGTACG1001.3929854E-436.2456784
GTCTCGC7200.032.4373441
CTGTCGC11500.030.892369
GTGTAAA11450.029.3210931
TCGCTCT9250.028.9965424
GTATAAG5150.028.343311
TTACGTT1306.490042E-427.8812894
GTCTTGC17500.027.108351
GTGTTAC7050.026.9160941
GTGTTAG13300.026.8888511
GTGCTAT7750.026.3684231
GTTAGCC11000.026.3616493
GGAGTGC45600.025.9153215
GTCTAAC4550.025.6647111
GTGTTAT8250.025.6549911
TATACTT5150.025.339565
GAGTGCA48550.024.939066
CGCTCTG9600.024.9216355
Produced by FastQC (version 0.11.5)