FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351740
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC959.896943E-538.4130971
TCTGTCG8300.035.8120578
TAGTCCG1301.656347E-533.4597135
GGTATAC3000.031.6267811
GTCTCGC7250.031.207331
GTATTAA5850.031.1901231
CGCCGTT2003.257246E-729.000269145
GTATTAT4900.028.3002621
TGTTACG1555.5055843E-528.0629842
GTCTTAC5500.027.8669591
GTCTTGC14900.027.4305631
GTAGCAC6800.026.6518233
GTGTTAG12150.026.430741
TCGCTCT8100.025.9531694
GTCTCAC18950.025.4195381
GTATTAG4350.025.1672041
GTGTAAG9200.024.5927311
GGGTAAT5650.024.5435891
CTGTCGC11250.023.843659
GTCGTAA1550.001740386723.5435141
Produced by FastQC (version 0.11.5)