FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238525
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7900.025.696358
GTCTCGC10350.021.6503261
CTGTCGC9850.020.9645889
TGTCGCC9850.020.96458810
TCGCTCT11300.019.8252664
GTATTAT7050.019.368691
TCTCGCT12100.019.0936722
GTCGCCC8850.018.98315811
CTCGCTC11700.018.549123
GTATAAG5200.018.1796361
CGCTCTG11500.017.6541825
GTATAAA8000.017.5063151
ATCTCGT7050.017.3754570
GTATTAG6900.017.2526021
GTATAGG4900.017.1490441
TACGAAC1250.001763824716.79957828
GTATTAA5900.016.6161651
TCGCCCA11000.016.5455512
GTCTTGC19750.016.3096831
ACGATCT7350.016.19032118
Produced by FastQC (version 0.11.5)