FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3789982
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.024.926918
GTAGATC17100.023.94586870
TGTAGAT32150.023.07786870
CTGTCGC12600.022.2222949
TGTCGCC12400.021.16914410
GTCTCGC11650.020.1394481
GTATTAT5950.020.0106261
GTACGGA1055.450202E-420.0106241
GTATTAA6600.019.6316361
GTCGCCC11650.019.52615511
GTTTACG900.00526287419.4547751
GTCGTAC950.00719151818.4308381
GTGTAGA42200.018.41116770
GTATCGA1150.001008667218.2606896
GTACGTA1000.00966476317.5092961
TGTACCG1200.001341928817.4998265
TCGCCCA13750.017.30777512
GTCTTGC20950.016.8824731
TCGCTCT13200.016.7043784
TCTCGCT13200.016.7041592
Produced by FastQC (version 0.11.5)