FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822738
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.024.361868
CTGTCGC12200.023.5237399
TGTCGCC11950.023.43011310
TCTCGTA6600.021.74248970
GTCGCCC11750.019.95672411
GTCTTGC24900.018.4204561
TCGCTCT11450.018.3437844
GTCTCGC11100.018.2950441
GTATTAG7700.018.1885341
CTAATCG1200.00134193317.49982344
TCGCCCA14600.017.0199312
GTATTAT7850.016.9489351
GTGTTAC8950.016.8218541
GTATAGA6700.016.7225931
GTATAGT7750.016.264071
GTGTAAT9750.015.8007071
GTGTATA9800.015.720091
ATTACTC7350.015.7170063
GTATAAA9000.015.5613011
GTGTTAT8850.015.4294261
Produced by FastQC (version 0.11.5)