FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643898
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA7650.028.36538970
TCTGTCG9200.027.7707488
ATCTCGT14250.025.05208270
GTCTCGC11300.024.4785651
TGTCGCC10850.021.28956210
CTGTCGC11550.020.3023059
TCGCTCT13200.020.1546694
CTCGCTC13350.019.9282113
TCTCGCT14100.019.8618092
TATCTCG18400.019.78217570
GTATAAG5750.018.2679881
GTATTAG7100.017.7533971
GTCGCCC10950.017.5792811
TATACGG1000.00968402817.503222
GTCTTGC20250.017.4635961
TTATACT6250.017.3627174
GTATAAT6700.017.2455271
TCCTACG2056.667342E-717.0763112
CGCTCTG13750.017.056985
TCGCCCA12650.016.60019912
Produced by FastQC (version 0.11.5)