FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643898
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.023.2085698
GTCGCCC9200.021.30319211
CTGTCGC10800.020.416689
GTAGATC11650.019.82752270
TGTCGCC10650.019.71832310
CGGTCGT950.00721350818.42106410
TCGCTCT10750.018.2328194
GTCTTGC19950.018.0806061
GTATAGA6200.018.0749441
GTCTCGC10350.017.9330521
TCGCCCA12250.016.85645912
GTTTCGC4000.016.6243253
GTATAAG6850.016.3598041
GTCTTAC9450.015.935121
TGTAGAT22650.015.7609570
CTCGCTC11450.015.5888873
GTCCGGT1350.0029369315.5645351
ACCGCGT1350.002948236215.5555658
GTGTTAA9500.015.4826171
GTACTAT4350.015.2961821
Produced by FastQC (version 0.11.5)