FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981871
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA802.471652E-630.62293267
TCTGTCG7600.025.7903258
GTCTCGC8750.024.8108061
GTAGATC12800.022.96758370
CTGTCGC9250.022.3250629
TGTCGCC9500.021.36912710
TCGCTCT11200.020.6253344
GTATTAG6150.020.496731
TCGCCGT1205.7245845E-520.41528766
TCTCGCT10500.019.6676462
TCGCCCA9750.019.02435712
GTCGCCC9050.018.94899711
GACTCGT1301.05822364E-418.846466
CGCTCTG11300.018.5843735
CTCGCTC11850.017.7203223
GTATTAA5350.017.6712471
TGTAGAT21350.017.37616770
TATACTT4750.016.9476455
CCGACGC2702.419256E-916.85099670
GTCTTAC7200.016.5349791
Produced by FastQC (version 0.11.5)