FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3866487
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA11000.032.13641770
TCTGTCG11300.025.0885378
ATCTCGT20050.024.96263370
CTGTCGC12900.022.7907379
TGTCGCC12850.022.60704210
GTCTCGC11550.021.5218681
GTATTAA5600.021.2566321
TATCTCG27150.021.01292670
GTCGCCC12300.019.91873211
TCGCTCT13550.019.6328054
TCGCCCA13300.018.68424212
TCTCGCT14650.018.3983152
GTCTTGC22750.018.1595121
CTCGCTC14600.017.7413623
GTACGAC1000.00968924917.5016143
GTATTAC6100.017.2184891
GTATTAG6200.016.940771
GTATAAG6450.016.8269561
CGACGCA1250.001763897216.79959328
CGCGATC7800.016.15366617
Produced by FastQC (version 0.11.5)