FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865128
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC21300.029.08311570
TGTAGAT39450.022.71120670
GTGTAGA47800.018.81684769
GTCTTGC24450.018.0455191
GTATAAC3750.017.7418961
GTATTAA6400.017.508451
GTATAAT6750.016.6006031
CTGTCGC11200.016.5621369
TCTGTCG9400.016.3826188
GTCTCGC10700.016.0357761
TACCCCG2503.1228956E-715.3996625
TCGCTCT12250.015.1425254
TGTGTAG60750.014.86348668
GTATTAC5050.014.5614821
TTGCTCT32000.014.2184384
GTGTTAC8650.014.1686881
GTATACG1750.001015000714.0067591
GTATAAG6250.014.0067591
GCGTAGA1750.001015000714.0067591
TACACCG1500.00593945113.9996925
Produced by FastQC (version 0.11.5)