FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589976
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8150.032.63785670
ATCTCGT14050.021.92158770
TCTGTCG8950.019.5529638
GTCTCGC9150.019.1338371
TGTCGCC9700.019.1238710
GTATTAT5750.018.877611
CTGTCGC10100.018.3662369
TATCTCG17150.018.16316469
TCGCTCT10450.018.088044
GTATAAT6450.017.9146121
GTATACG1000.00967049817.5074631
GTACTAA4850.017.3269711
GTATAAA8450.016.9894891
GTGTAAG10150.016.9037571
GTCTTGC20550.016.8685021
GTCGCCC9350.016.8450611
TTATACT7800.016.6043224
GTATTAG6500.016.1607341
CTCGCTC10850.016.130743
GTATAGA5650.016.1130621
Produced by FastQC (version 0.11.5)