FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589976
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10000.021.3635431
GTAGATC12100.021.11467770
TCTGTCG9400.020.8509248
GCGCGAC1107.484499E-419.08971616
GTCTTGC20550.019.0875261
TGTCGCC10200.018.87189710
CTGTCGC11100.018.603489
TCTCGCT11300.018.5857582
GGGTACG1602.5957339E-517.51111
TCGCTCT11800.017.4996384
CTCGCTC11450.017.4227353
TCGCCCA11050.017.10300412
GTCGCCC10100.016.63262411
GTCTTAC9900.016.2729431
CGGATAA1300.00229196216.15396140
CGCTCTG11650.015.9224175
GGCTAGA12100.015.9191831
GTCCGGA2009.915515E-615.7599931
GTATTAC6000.015.7599911
TGTAGAT22300.015.38041970
Produced by FastQC (version 0.11.5)