FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388145
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.027.513728
ATCTCGT12550.026.77338470
GTATTAT5650.024.787981
CTGTCGC10250.023.9018049
TGTCGCC10250.022.53598810
TCTCGTA6700.021.94068770
GTCTCGC10150.021.3872641
GTCTTGC21300.020.2187861
GTCGCCC9600.019.68697711
GTATAGG5300.017.8368231
GTACTAG4150.017.7174321
GTCTCAC24050.017.6156981
TCGCTCT11400.017.501864
TCGCCCA11600.017.49953512
GTGTTAG16050.017.4519731
TCTCGCT11850.017.4292982
CGCTCTG11000.017.1836455
GCGAATA2651.886292E-917.17011670
CGAATCT28800.016.4065470
GTATACG1300.002286350616.1598551
Produced by FastQC (version 0.11.5)