FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388145
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT6250.026.3326131
GTCTCGC10450.024.461481
GTATACG1301.6021295E-724.2423841
TCTGTCG9750.023.3324588
TCGCTCT11600.022.024714
CTGTCGC11500.021.6078839
TGTCGCC11850.021.56005510
TCTCGCT12000.021.0001412
CTCGCTC12300.019.3482973
GTATTAG7650.019.2248951
GTCGCCC11500.018.868311
GCTATCG950.00721499318.4203628
GTATAGA5750.018.269621
TCGCCCA12500.017.91906212
GTCTTGC19200.017.5083871
GTATTGG7200.017.5083851
TGTAGAT21200.017.49882770
CGCTCTG12350.016.720025
GTACGAT1503.149888E-416.3411621
GTCTAAT5600.016.2577881
Produced by FastQC (version 0.11.5)