FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374264
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7250.027.998568
GTCTCGC10050.024.7423291
CTGTCGC9000.024.109879
TGTCGCC9900.021.91806410
TCGCTCT11150.021.3441944
GTCGCCC9300.020.32093411
TCTCGCT12100.019.3817542
CTCGCTC12050.018.8784183
CGCTCTG11550.018.7869135
CGACCGT1150.001008415518.26128449
GTCTAGC8950.018.0004391
GTATTAA6050.017.945461
GTATTAG6900.017.7650831
GTATAAG6100.017.2242241
TCGCCCA10650.017.0880712
TTCGCCG1653.398178E-516.97008144
GTGTAAA11850.016.550761
GTGTAAG10550.016.2664171
CGCCGTA1957.850671E-616.15253668
GTCTTGC21000.016.0103281
Produced by FastQC (version 0.11.5)