FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521448
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.026.8331938
CTGTCGC11250.023.9554319
GTCTCGC10600.023.788661
TGTCGCC10950.022.37431110
GTCGCCC10900.020.22781611
TCGCTCT11950.020.2090994
GTATATA7600.019.354371
GTAGATC13200.018.55945670
GTATTAG7550.018.5548061
CGCTCTG11750.018.4679875
CGTGTAG28800.018.22777769
TCTCGCT12450.017.9939172
GTGTAAA12200.017.5110971
ACCGCGT1401.8671331E-417.4999078
TCGCCCA12250.017.42724212
GTGTAGA31000.017.38601770
GTATTAT5700.017.2038841
GTCTTGC19400.016.4279371
CTCGCTC13250.016.376113
GTATTAA7100.016.2779221
Produced by FastQC (version 0.11.5)