FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983219
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7650.026.9929628
GTATAGA4600.022.832551
TCGCTCT9950.022.162994
GTCTCGC9100.021.9292831
GTGTATA7350.021.4346371
TGTCGCC9400.021.22300710
CTGTCGC9300.021.0748759
TACGCTT1205.7172678E-520.418685
CGCTCTG9900.020.1535035
CTCGCTC10400.019.5211563
GTATTAT4600.019.0271241
GTCTTGC17850.019.0249921
GCGTTAC1150.001006616418.2660391
GTGTTAG13050.018.242711
GTCGCCC9500.018.05229411
GTATTAG6450.017.9120461
GAGTATA4300.017.9120461
GTATAGG4500.017.8939531
TTATACT4900.017.8589044
GTGTAAC5950.017.6520561
Produced by FastQC (version 0.11.5)