FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939439
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7050.023.829988
TAAGTCG1202.1694214E-623.3351085
GTCTCGC7450.022.086351
TACGCAA1454.613321E-721.72431210
CTGTCGC7650.021.0459219
TCTCGTA3950.020.37991170
GTCTTGC18250.020.1422881
TGTCGCC7800.019.74374810
TCGCTCT8250.019.5166364
GTCCGAT900.005278537519.44460112
CGTTTCG950.007212875418.4212029
GTCGCCC7650.018.30080211
GTGTTAG11100.017.6623061
GTATTGG5800.017.5046061
CTCGCTC9050.017.4046383
ATCTCGT6500.017.23090770
CTACGCA2451.33059075E-817.1429949
GTATTAA6600.016.9741651
TAATACT5250.016.6679344
GTCTTAC7600.016.5833111
Produced by FastQC (version 0.11.5)