FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594218
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG703.4807854E-530.0147951
GTCGTAC604.97163E-429.1810511
GTCTCGC7600.026.72371
CTGTCGC8400.025.8321239
TCTGTCG8250.025.4533528
GTACGAA700.001223449425.012331
TGTCGCC8650.024.27585210
GTCGCCC8700.022.92907511
TCGCCCA9500.020.99820712
TCGCTCT8400.020.8323574
GTATAAG4650.020.3326021
TCTCGCT9350.020.2149072
TACGGGT1408.2611605E-619.9990634
GTCTTGC14750.019.9420321
TCCGCTA900.005280045319.44353533
CTCGCTC9300.018.8163223
CGCTCTG8400.018.7491235
CGCCGTA1501.4975138E-518.66687262
GTATAAA4950.018.3929061
GTCGTAG1150.001005150318.2698751
Produced by FastQC (version 0.11.5)