FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300831
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA6600.031.81802470
TCTGTCG9100.022.6921948
CTGTCGC9950.021.4571789
AACGCCG850.003790358520.5900065
GTATTAG6700.019.3329471
ATCTCGT11050.019.3211769
TGTCGCC10700.019.2989710
GCGCGTT950.00721422918.42068141
GTCTCGC10100.017.3308491
GTCGCCC10300.017.33001111
ATAGACG1452.4391773E-416.898263
GTCTTGC18950.016.4418981
TCGCTCT11750.016.3846364
GTATAGT5100.015.7880611
GTATAAA7950.015.4124641
GGTCCGC2051.2576349E-515.3695031
GTATTAT5850.014.9608171
TCGCCCA11700.014.957190512
TATTAGG6350.014.8836182
GTATTAA5450.014.774151
Produced by FastQC (version 0.11.5)