FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300831
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.025.5608468
GTCTCGC10350.023.6835331
CTGTCGC10800.023.6565289
TGTCGCC10800.023.65652810
TCGCTCT11000.023.2260554
TAGTCGT850.003793063320.587476
GTAGATC10900.020.5490770
GTCGCCC10550.019.57213811
TAAATCG900.005280053719.443725
TCTCGCT13100.019.2370912
CGCTCTG11300.019.2028265
CTCGCTC12100.018.8003853
CCGTGTA26100.018.63858870
TCGCCCA11200.018.43597612
CGCCGTA950.00721557318.42008867
GTACCGC1150.001005126318.2701551
GTCTTGC20350.017.7239931
GTGTTAC7200.017.5088981
CGTGTAG19300.016.68281270
GTGTTAG14300.016.4069391
Produced by FastQC (version 0.11.5)