FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301608
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA10700.036.6351170
ATCTCGT20400.028.30851270
GCGTAAC700.001224198525.009951
TCTGTCG9450.024.0741738
CTGTCGC10200.023.333439
TGTCGCC10450.022.44028310
GTATTAT4900.022.151671
ATACCCG1506.385126E-721.0026324
GCGTAGT1558.726038E-720.3306711
GTCTCGC10300.020.0565221
GTCTTGC18700.019.8474671
TACCCGT1601.1863649E-619.6899665
GTCGCCC10700.018.31783311
TAATACT4600.018.2631574
GTATTGG5600.018.1322141
TCGCTCT10850.018.0667784
AATCTCG32750.017.63339269
CGTTTCG1401.8669544E-417.500079
TCTCGCT12850.016.617372
GTATTAG6000.016.3398341
Produced by FastQC (version 0.11.5)