FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086130
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA6900.029.92798670
TCTGTCG8200.028.5970638
GTATCGA700.001227147924.99956511
GTCTCGC9850.023.8151821
CTGTCGC9850.023.8066949
TGTCGCC10200.023.33292810
GTCGCCC9750.022.9739611
TCGCTCT10600.021.4646744
GTATAAG4800.020.4235921
TCGCCCA11300.020.13239512
ATCTCGT10500.020.00030169
GTCTTGC19100.019.9805971
GTATTAT4500.019.451041
CGCTCTG11550.018.4866245
TCATACG950.007214010718.4207329
TCTCGCT12300.018.2137222
CTTCGTA1551.9866622E-518.0642039
TTATACT4300.017.9089874
GTATAGA5950.017.6530461
CTCGCTC12050.017.4293423
Produced by FastQC (version 0.11.5)