FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597958
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42940.16528365739553913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC19250.025.45282470
TCTGTCG7300.021.575138
GTATAGA2650.021.1416321
GTATTCG1003.9234757E-421.0094971
TGTAGAT29000.020.39517270
GTATAAG3100.019.2022291
GTCTCGC9750.019.0342451
CGACGAA950.007215805418.41980670
GTCCGGA1551.9784571E-518.0726851
TCGCTCT10100.018.0192764
TGTCGCC9150.017.97796210
GTCTTGC15200.017.9686471
TACCCGA1200.001341947117.499495
GTCGCCC8850.017.39995411
GTATTAT3307.2759576E-1216.9773711
GTGTAGA34900.016.84699869
CTGTCGC9650.016.6837739
GTATTAG3800.016.5864451
TCGCCCA10150.016.55060612
CGCCGTA3401.0913936E-1116.46979167
Produced by FastQC (version 0.11.5)