FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226539
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.028.533928
CTGTCGC9200.025.8685579
TGTCGCC9350.023.95628510
GTCGCCC9050.022.81643511
GTACCGC1454.5813795E-721.7401371
GTCTCGC9150.021.0537451
GCGTAAT850.003776088620.6033971
CGCCGTA900.005279608519.44398967
TCGCCCA10450.019.42508112
GTATTAG6500.019.3988911
TCGCTCT9800.018.928134
ACGACGT950.007214979318.4203365
GTCTTGC19900.018.3049281
GTACGGG1000.00965305617.5128881
GTCCGTA1653.377368E-516.9821951
GTGTAAC6550.016.5770851
CGCTCTG10650.016.4312845
TTGCTCT23700.016.392024
GTATTCG1503.143666E-416.3453621
GTGTCGC2151.0904732E-616.2910581
Produced by FastQC (version 0.11.5)