FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125021
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5150.023.10685570
TCTGTCG7400.020.337898
GTCTCGC8700.019.7186891
ATCTCGT9000.019.44449470
TCGCTCT9650.018.8613594
GTCTTGC18750.018.4856641
GTATAGA4850.018.0467681
CTGTCGC9050.017.79019
GTATAAT4950.017.6821881
GTCTTAC7050.017.3812141
GTATAAG4650.017.3171371
CGCTCTG8500.017.2952695
TGTCGCC9100.016.53850410
GCGTTAG1300.00228729516.15881
GTATAAC2906.21003E-915.6944661
GTCGCCC8700.015.68969411
CTCGCTC11350.015.4195283
GTATTAG6150.015.3705651
TCTCGCT11150.015.0687482
GTCTAAA6300.015.00461
Produced by FastQC (version 0.11.5)