FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125021
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.023.3324418
CGCCGTA1102.9263996E-522.27187568
GTCTCGC9850.021.684361
TCGCTCT10350.021.3035344
TGTCGCC9350.020.58744810
CTGTCGC8750.020.3992219
GTCGCCC9050.018.56223311
TCGCCCA10200.018.52840812
CTCGCTC11000.017.8169333
CGCTCTG10700.017.3357875
GTGTTAC6350.017.0938931
GTACTAA3950.016.8426151
GTACTAG2954.3473847E-1016.6172471
GTCTTGC19750.016.4880331
TATAATA4050.016.4196532
TCTCGCT12550.016.1751982
AACGAAG6750.015.03694432
TAGTACT3503.0559022E-1014.9994284
GTGTAAT7250.014.9718911
GTCTAGC8750.014.8063051
Produced by FastQC (version 0.11.5)