FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794740
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.047.064878
GTCTCGC14600.042.1155971
TCGCTCT14750.039.5386124
CTGTCGC15600.036.4631589
CTCGCTC16900.036.212683
GTCTTGC27000.036.1698651
TCTCGCT17600.034.7724042
CGCTCTG17000.031.7638425
GTATCGC1050.007794491527.5579951
GTACTAC4500.027.328341
GTATTAT8000.026.2231521
GGTATAC5400.025.4528681
TTGCTCT36150.025.0952014
GTCTCAC32900.025.0660461
GCGTTAC1450.001242923924.9447351
GTATACG1751.2755345E-424.802191
GTGTAAT13000.024.4842171
GTATTAC7050.023.6001951
ACCGTGT8600.023.4412238
GTCCTAC8350.023.391291
Produced by FastQC (version 0.11.5)