FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794740
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10500.054.150761
TCTGTCG11900.045.375218
TCGCTCT14850.041.6942674
TCTCGCT15700.036.2412152
CTCGCTC16500.036.217133
CGCTCTG15950.032.950845
GTCTTGC23300.032.2240831
CTGTCGC18200.029.6684069
GTATTAG7950.029.3414861
GTACTAT6600.028.7163121
GGTATAC5500.027.8327351
GTGTTAG17100.027.7087231
GTATTAA8100.026.9982431
GTATAGG7100.023.6139551
GTCTCAC30300.023.0955261
ACCGTGT9100.022.152418
TAGTACT5600.021.8557264
GTGTAAT10500.021.5214561
GTCTAGC11250.021.3826071
GGAGTGC64250.021.2904475
Produced by FastQC (version 0.11.5)