FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061708
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12050.046.603378
GTCTCGC13850.040.2231981
GTCTTGC30800.038.9935721
TCGCTCT14250.037.3881264
CTGTCGC16450.037.202929
TCTCGCT16900.030.6739562
CGCTCTG17300.029.5475885
GTCTCAC36750.028.5460681
TTGCTCT38700.028.464094
GGTATAC5650.028.1714951
CTCGCTC18300.027.9334243
GTGTATA13500.027.8679621
GTGTAAC9150.026.8839741
TCTTGCT43400.026.0455722
GTATAAT8350.025.993841
CTTGCTC39500.025.3357983
TCTGTTG32500.024.5894838
GGGTTTC50050.024.574261
GTATTAA6000.024.1165071
GTCTTAC12050.023.4160251
Produced by FastQC (version 0.11.5)