FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061708
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13550.038.7875868
GTCTTGC26600.037.2517551
GTCTCGC13800.035.3740841
TCGCTCT15050.031.5740854
GTGTTAG21200.028.5253941
GTATTAG9900.027.9664841
CTGTCGC20350.027.2417839
TCTCGCT16450.026.7068162
GTTAGCC17200.025.9529463
GTTTCAC43300.025.4405753
CGCTCTG18400.025.0429525
ACCGTGT9800.024.978258
TTCACCG9600.024.7494815
GGGTTTC48350.024.412261
TAATACT7100.024.3375174
TGTTAGC19050.024.1959782
GTCTCAC32550.024.1747631
CTCGCTC17900.024.1335743
CTTGCTC36050.023.966213
GTATAAT8850.023.8750421
Produced by FastQC (version 0.11.5)