FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067939
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13350.039.8815231
TCTGTCG13650.038.502148
GTCTTGC27450.033.212211
TCGCTCT15600.031.8438974
TAATACG1851.7495586E-731.1329294
GTGTTAG21850.029.7076761
TCTCGCT16550.027.8477382
GTATTAC7600.027.8300861
GTATTGG8800.027.3502561
GTATTAG9600.025.83081
CTGTCGC19800.025.8159339
CTCGCTC18250.025.2475263
CGCTCTG17450.025.167275
TTAGCGC2051.36328545E-524.5836263
GTTAGCC17600.024.5437163
GTATTAA7400.023.6542781
GTATAAG9000.023.5009611
GTATTAT8700.023.4730171
TATACTG8650.023.3047085
GTCTCAC34000.022.9527651
Produced by FastQC (version 0.11.5)