FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838599
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12550.040.9389151
TCTGTCG10750.040.8532458
TCGCTCT13600.038.1165964
GTCTTGC24800.034.431191
TCTCGCT15050.032.051712
CTGTCGC15350.030.4893679
CTCGCTC15250.030.2155593
GTGTAAA16450.027.7138161
CGCTCTG16350.026.8606975
GTATAAT7550.026.8369271
GTCTTAC11900.026.7563841
GTACTAC4500.025.7293721
GTCTAAT6000.025.3273491
GTGTAAT11300.024.9751381
GTCTCAC33000.023.9019991
TTCACCG8200.023.7058285
TTGCTCT36900.023.4139864
TAATACT9000.023.1993584
GTATTAG9100.023.061011
GTGTTAG18950.022.9120391
Produced by FastQC (version 0.11.5)