FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867815
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.040.34398
GTCTCGC12500.039.3613241
GTCTTGC27050.036.913281
TCGCTCT13850.034.309684
TCTCGCT15450.031.6891462
CTCGCTC15750.030.6278673
CTGTCGC15200.030.3129799
GTCGTAC1204.1076858E-430.1480731
GGTATAC5850.028.4474121
GTATCGC1050.007786305627.5639511
CGCTCTG16250.027.0270715
TTGCTCT36700.026.0920434
GTACGAT1450.001241365924.950131
GTATTAT8750.024.8075581
GTGTTAC11750.024.6316181
GTCTCAC34400.024.6092411
CTTGCTC36950.024.5515333
GTTTCAC45100.023.7865923
GGGTTTC48100.023.7674621
GTATAGC6800.023.4090921
Produced by FastQC (version 0.11.5)