FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867815
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13350.033.975658
TCGCTCT12650.032.4420284
GTCTTGC26600.032.076271
GTCTCGC13200.031.4905741
TAGCGCG1000.00628588228.7994127
GTATAAC5700.026.8673041
CTCGCTC14400.026.499463
GTCTCAC34650.025.2555981
GTGTTAG17400.025.1467381
CTGTCGC18600.024.385759
TCTCGCT16200.023.5628832
TTGCTCT38200.023.559734
GTATTAG8400.023.4403511
ATACGGC1550.001883851823.2253343
GTGTAAT11650.023.1609021
GGAGTGC69700.022.8288045
GTACTAT6150.022.529841
CGCTCTG15050.022.4845945
GTATACG1952.514803E-422.4386271
GTACGGT1952.514803E-422.4386271
Produced by FastQC (version 0.11.5)