FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866345
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13650.044.0191151
TCTGTCG11450.044.0152248
TCGCTCT14350.039.1400384
GTCTTGC24050.036.42221
CTGTCGC15000.035.999429
TCTCGCT15900.034.4193422
CTCGCTC17300.032.049643
CGCTCTG16950.031.4320265
GTACTAG5250.027.5782411
GTATTAT8050.026.9787121
GTATAAT8600.026.9368881
TAATACT8150.026.5058774
TCGATCT11150.025.829189
CTCGATC11000.025.525978
TAGTACT5950.025.414464
GTTTCAC40250.025.0461353
TCTCGAT10950.024.9850277
GTATTAA8200.024.7195221
GGGTTTC45600.024.6072311
GTCGTAC1801.536583E-424.1309621
Produced by FastQC (version 0.11.5)