FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692224
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.046.6493578
GTCTCGC13650.036.5672261
CTGTCGC15650.034.0445379
TCGCTCT13050.033.6581084
TCTCGCT15250.032.107122
CTCGCTC15300.030.5909023
GTCTTGC25100.029.9733431
CGCTCTG15150.026.6146985
TTCACCG7750.026.013725
GTTTCAC40450.025.8118923
GGGTTTC44650.024.6262171
GTGTTAC10100.024.3519171
GTATTGC7850.023.9595851
GTGTTAG19000.023.6055261
GTATTAG8300.023.5321311
TCACCGT9500.023.4954766
TATACTG8000.023.4007385
GTGTTAA10000.023.1486471
TCGATCT11200.023.1427259
AACTTCG2202.352628E-522.9098117
Produced by FastQC (version 0.11.5)