FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692224
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11450.041.4312171
TCGCTCT12900.037.952694
TCTGTCG13250.032.602488
GTCTTGC26100.031.8774971
TCTCGCT15100.030.5238512
CGCTCTG14750.030.263775
CTCGCTC14800.029.6750533
TTGCTCT32800.028.7554934
GTATTAA9100.028.0702631
GTGTTAG18400.027.3685051
GTATTAG8000.026.4562241
GTGTTAT9500.025.3518811
GTACTAG6050.025.3328341
GTACGGT2051.2265798E-524.9209181
GTATAAT8250.024.7698821
GTTAGCC15100.024.3173683
GTATTAC6350.024.1360051
GTACCGA1500.001556588623.9994966
CTGTCGC18300.023.999059
GTCTCAC31250.023.354461
Produced by FastQC (version 0.11.5)