FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881894
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.044.3927848
GTCTCGC12500.037.6357771
GTCTTGC27150.037.587791
TCGCTCT14900.035.274434
CTGTCGC16750.033.0995379
ACGTTCG900.003755471731.9982195
GTATAAT7250.030.947191
TCTCGCT16100.029.9621072
CTCGCTC16750.029.6590793
GTCTCAC31150.029.0435371
TTGCTCT37050.028.5662024
CGCTCTG17200.028.044955
GGCTAGA17200.025.6684071
TAGTACT6500.024.368724
TCTTGCT39600.024.181312
TCTGTCA37250.024.1597298
GTATTAG9300.024.1254961
CTTGCTC37400.024.0636673
GTGTAAC8150.023.977491
GTATTAA8250.023.6868521
Produced by FastQC (version 0.11.5)