FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881894
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12400.041.169451
TCTGTCG13200.037.6349648
GTCTTGC26100.035.7658161
TCGCTCT14650.035.384354
CGCTCTG17650.028.5541785
TCTCGCT18150.028.1720642
TTGCTCT36250.028.0044754
GTATTGC7100.027.7334631
CTGTCGC19050.027.2115849
CTCGCTC18000.026.7990043
CGTTGCG1400.001041343125.713339
GTATAGT6700.025.0352381
GTCTCAC31500.025.0041371
GTGTTAA11100.024.9665951
CTTGCTC35400.024.6092533
TCTGTCA35500.024.5399328
GTTTCAC41450.024.3175493
GTGTTAG18600.024.3095781
GGGTTTC45150.024.2288151
GTGTTAC10650.023.967191
Produced by FastQC (version 0.11.5)