FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957801
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.043.9230588
GTCTCGC12850.042.2536281
GTCTTGC31200.037.1254121
TCGCTCT13800.034.9585154
CTGTCGC17700.032.9482469
TCTCGCT15400.031.326462
GTATTAG9750.029.700331
CTCGCTC16400.028.5382163
TAATACT9150.028.3294874
CGCTCTG17850.025.8152895
TTGCTCT41300.025.2798944
CTTGCTC39600.025.092343
GTGTTAG18900.024.8975961
GTATAAC5550.023.4793151
GTGTTAA12350.023.4476281
GTACAGA15950.023.14811
GGTATAC5050.022.9368881
TCTGTTG32500.022.8186078
TCTTGCT45050.022.696082
GTATTAT9150.022.1535221
Produced by FastQC (version 0.11.5)