FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17250.037.9799778
CTGTCGC22100.030.2965439
GTCTCGC19500.029.9359441
GTCTTAC15400.028.5464211
TCGCTCT21150.027.9129564
GTCTTGC38500.027.8912561
TCTCGCT23150.024.9047152
TTCACCG12200.024.7851685
GTGTAAC12800.024.2103481
GTGTTAG28650.024.1484431
GTCTCAC47050.023.8950271
CTCGCTC24350.022.4935073
CGCTCTG24300.021.3318565
GTATAAT11500.021.3070661
GGGTTTC68200.021.2399861
CCGTGTT16450.021.0076629
TACACTG23650.021.0048875
GTATTAT12700.020.9961931
GGAGTGC96750.020.984595
GTTTCAC62700.020.9192643
Produced by FastQC (version 0.11.5)