FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG19550.036.4591068
GTCTTGC39200.034.2096941
GTCTCGC19250.033.7080881
CTGTCGC25550.030.433039
TCGCTCT22700.028.8624464
GGTATAC8150.027.4237231
TCTCGCT23600.026.876392
GTATTAG13200.025.125011
GTCTTAC17850.024.2345711
GTATTAT13450.023.5859151
CTCGCTC26350.023.4982473
CGCTCTG25200.023.427775
GGGTTTC68100.023.0797821
GGAGTGC97800.023.0421565
GTCTCAC48500.022.8929271
GTGTAAC12050.022.7362521
TTGCTCT54700.022.5074564
GTATTAC10400.022.1839521
GAGTGCA107050.022.127226
GTTTCAC61900.021.7504653
Produced by FastQC (version 0.11.5)