FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267254
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.044.737278
CTGTCGC17250.037.5605289
GTCTTGC33250.035.985981
GTCTCGC15750.031.1204091
GTATTAT10150.027.69591
TTGCTCT46600.026.8807624
CTTGCTC43200.024.6941973
GTTTCAC51750.024.6535723
GTGTTAC13550.024.4700851
TCGCTCT16500.024.4333134
GGGTTTC59050.024.2912641
GTATAGA10850.023.9160631
CGCTCTG17600.023.7243085
GTGTTAG23050.023.453481
TCTCGCT17900.022.9529632
GTATTAC8950.022.550281
GTCTTAC11400.022.1299341
GTATTAA8800.022.1155641
GTATTAG10900.021.8224931
GTACTAC6350.021.5673521
Produced by FastQC (version 0.11.5)