FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267254
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14250.039.4108778
GTCTCGC13050.039.2099651
CTGTCGC18200.031.645249
GTCTTGC30700.030.0483231
TCGCTCT16100.027.7269544
TCTCGCT16600.026.9156952
GTGTTAC12200.026.5828361
CTCGCTC17250.025.0437013
GTATTAC7250.024.8513851
TACACTG22000.024.5456715
GTATTAT11400.024.0230081
CGCTCTG16900.023.8581985
GTGTAAG15500.023.7130321
TACACCG2752.1075175E-723.5638455
GTATTAA10950.023.0357591
GTATAAG9550.022.6394841
GTCTCAC38950.022.018521
GTACTAC5950.021.8023951
TTATACT10400.021.461734
TGGACCG5051.8189894E-1221.3863265
Produced by FastQC (version 0.11.5)