FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782926
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15150.033.2648478
GTCTCGC16500.032.3256761
GTCTTGC36450.030.254761
GTATTAG12600.028.602191
GTCTTAC11900.027.8618971
TCGCTCT19050.027.5884954
GGTATAC6550.027.5105021
CTGTCGC19550.026.8829089
TCTCGCT20850.024.8900782
GTGTAAC11350.024.7667241
CGCTCTG20350.024.7647385
GTTTCAC59550.023.1180633
GTGTTAC15000.023.0648041
GGGTTTC65000.022.3994751
GTCCTAC12900.022.3496191
GTATAAT10400.021.4846441
TTGCTCT47450.021.3935574
GTGTAAA18700.021.199271
GTGCTAA16350.020.719531
GTATAGG10800.020.6889151
Produced by FastQC (version 0.11.5)