FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782926
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16600.042.070868
GTCTCGC17950.038.1610531
GTCTTGC36750.033.7467771
CTGTCGC21650.031.2586679
TCGCTCT19750.030.9862884
TCTCGCT23500.028.220222
ACCGTGT11650.026.5742058
CGCTCTG22550.026.500215
GTTTCAC59400.025.8173053
GGGTTTC66350.024.9947071
CTCGCTC25450.024.3292333
GGTATAC7450.024.1960721
GTGTTAC15950.023.9594231
TTGCTCT49250.023.5362554
TTCACCG12200.023.0156125
GTGCTAC16100.022.392641
GTACTAT8900.021.8743361
GGTTTCA66850.021.6738012
CTTGCTC49350.021.591973
TACACTG23200.021.4130655
Produced by FastQC (version 0.11.5)