FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826903
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17900.045.8506858
GTCTCGC18000.043.2475931
CTGTCGC21500.039.847639
GTCTTGC34900.036.1429251
TCGCTCT20700.035.127324
TCTCGCT21750.034.1341172
CTCGCTC23650.028.9536343
CGCTCTG22400.028.9260065
TTGCTCT50800.028.6273824
GTATTAG12200.024.8141921
CTTGCTC51650.024.421843
TCTGTCA49650.024.3603788
GTCTCAC45850.024.2098481
GTGTTAG27500.024.1138081
TAATACT10000.023.7578954
GGTATAC8150.022.9946291
GTTAGCC19500.022.9175263
GTATTAA11650.022.8921431
GTATTAC7400.022.403031
GTTTCAC51250.022.2215253
Produced by FastQC (version 0.11.5)