FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826903
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC35300.042.0720831
GTCTCGC18100.039.0345341
TCTGTCG16800.035.56928
TCGCTCT20600.034.6002244
CTGTCGC22400.030.2112069
CGCTCTG22500.030.0785085
TATACCG1453.663123E-529.7916265
TCTCGCT23250.028.5183452
TTGCTCT49600.028.1598934
CTCGCTC25400.026.92783
GGGTTTC61700.026.6410391
GTTTCAC57000.026.2723793
GTGTAAG17600.026.2161431
GTCTTAC14750.025.9051041
CTTGCTC49550.025.8634993
GTCTCAC47400.025.7045421
TAATACT10650.025.6888664
TCTTGCT54200.025.3976522
GTATAAC6650.024.9349021
ACCGTGT14100.024.5091028
Produced by FastQC (version 0.11.5)